Animal Development I: Fertilization & Cleavage

Learning Objectives

  • List the sequence of early events in animal development (fertilization, cleavage, gastrulation, and organogenesis)
  • Explain the significance and describe the features of fertilization and cleavage in early animal development
  • Differentiate between intrinsic and extrinsic factors determining cell specialization
  • Explain and differentiate between the roles of cytoplasmic determinants and induction in determining body axes during early animal development

The information below was adapted from Khan Academy “Introduction to development.” All Khan Academy content is available for free at www.khanacademy.org

The purpose and stages of development

Your body consist of over 30 trillion cells, but you began as a single cell: a fertilized egg, or zygote. How did you become the large, organized multicellular individual that you are today? Development!

As an animal embryo develops, its cells divide, grow, and migrate in specific patterns to make a more and more elaborate body (plant cells perform differential expansion instead of migration). To function correctly, that body needs well-defined axes (such as head vs. tail). It also needs a specific collection of many-celled organs and other structures, positioned in the right spots along the axes and connected up with one another in the right ways. How are all of these complex processed accomplished and coordinated? They occur via four essential stages in early animal development:

  • Fertilization: the process of a single sperm cell combining with single egg cell to form a zygote.
  • Cleavage: rapid, multiple rounds of mitotic cell division where the overall size of the embryo does not increase.  The developing embryos is called a blastula following completion of cleavage.
  • Gastrulation: the dramatic rearrangement (movement) of cells in the blastula to create the embryonic tissue layers.  These tissue layers will go on to produce the tissues and organs of the adult animal.
  • Organogenesis: the process of organ and issue formation via cell division and differentiation.

Gastrulation and organogenesis together contribute to morphogenesis: the biological processes that result in an organism’s shape and body organization.

For this class session, we will discuss the first two steps above, fertilization and cleavage. We will carry on with gastrulation and organogenesis in the next class session.

The information below was adapted from OpenStax Biology 43.6

Development Step 1: Fertilization

Fertilization is the process in which a single haploid sperm fuses with a single haploid egg fuse to form a zygote. The sperm and egg cells each possess specific features that make this process possible:

The egg is the largest cell produced in most animals species. Human eggs are approximately 16 times larger than a human sperm cell. The eggs of different species contain varying amounts of yolk, nutrients to support growth of the developing embryo. The egg is surrounded by a jelly layer, composed of glycoproteins (proteins that have sugars stuck to them), that releases species-specific chemoattractants (chemical-attractors) that activate and guide sperm to the egg. In mammals, this layer is called the zona pellucida. The zona pellucida/jelly layer is separated from the egg by a membrane called the vitalline envelope, which is outside of the cell’s plasma membrane. Just underneath the egg’s plasma membrane are cortical granules, vesicles containing enzymes that will degrade the proteins that hold the vitalline envelope around the plasma membrane when fertilization occurs (more on this below).

This diagram represents the components of a mammalian/human egg cell prior to the process of fertilization. It shows the outer jelly coating, the plasma (cell) membrane with sperm cell receptors and the cortical granules just inside the plasma (cell) membrane. Image credit: By Chippolito – Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=18851511

The sperm is one of the smallest cells produced in most animal species. The sperm consists of head containing tightly packed DNA, a flagellar tail for swimming, and many mitochondria to provide power for sperm movement. The plasma membrane of the sperm contains proteins called bindin, which are species-specific proteins that recognize and bind to receptors on the egg plasma membrane. In addition to the nucleus, the sperm head also contains an organelle called the acrosome, which contains digestive enzymes that will degrade the jelly layer/zona pellucida to allow the sperm to reach the egg plasma membrane.

Sperm cells are divided into a head, containing DNA; a mid-piece, containing mitochondria; and a tail, providing motility. The acrosome is oval and somewhat flattened. By OpenStax College – Anatomy & Physiology, Connexions Web site. http://cnx.org/content/col11496/1.6/, Jun 19, 2013., CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=30132983

To ensure that the offspring has only one complete diploid set of chromosomes, only one sperm can fuse with one egg. Fusion of more than one sperm with an egg, or polyspermy, is genetically incompatible with life and results in zygote death. There are two mechanisms that prevent polyspermy: the “fast block” to polyspermy and the “slow block” polyspermy. These and the other steps of fertilization are described here:

  1. Sperm is attracted to and contacts the jelly layer/zona pellucida.
  2. Interactions receptors on the sperm cell and egg glycoproteins initiate the acrosome reaction. Digestive enzymes are released from the acrosome, and they destroy the jelly layer/zona pellucida thus creating a pathway for the sperm to approach the egg.
  3. The sperm reaches the egg plasma membrane and the sperm and egg plasma membranes fuse. This interaction is mediated by the sperm bindin protein, which binds to bindin receptor proteins on the egg plasma membrane. This interaction is species-specific, with slightly different versions of the bindin protein and the bindin receptor proteins in every different species.
  4. Sperm-egg membrane fusion initiates electrical depolarization of the entire egg plasma membrane, temporarily (for 10-20 seconds) preventing any other sperm from fusing with the egg plasma membrane. This membrane depolarization, mediated by an influx of sodium ions, is the fast block to polyspermy.
  5. Membrane depolarization by sodium influx initiates a cross the wave of calcium released across the plasma membrane.
  6. The calcium wave initiates the cortical reaction in the egg: the cortical granules fuse with the egg plasma membrane, releasing digestive enzymes that degrade the bindin receptor proteins that serve as sperm docking sites, and the proteins that hold the vitalline layer against the plasma membrane.
  7. The cortical reaction results in lifting away of the vitalline layer away from the egg plasma membrane, to create the fertilization envelope. The fertilization envelope is a barrier that prevents additional sperm from reaching the egg, and is the slow block to polyspermy.
  8. These events culminate in egg activation, causing the egg to recognize that fertilization has occurred and resulting in initiation of development.

Fertilization is the process in which sperm and egg fuse to form a zygote. The acrosomal reaction help the sperm degrade the glycoprotein matrix protecting the egg and allow the sperm to transfer its nucl eus.Image credit: LadyofHats Public Domain, https://commons.wikimedia.org/w/index.php?curid=696998, based on diagrams found here: http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2005/Dresser/review%20paper.htm, http://academic.brooklyn.cuny.edu/biology/bio4fv/page/actin.html, http://www.luc.edu/academics/depts.shtml.

The video below provides a nice overview of sex cells and fertilization, including the acrosome and cortical reactions:

And this video shows what happens in sea urchin eggs immediately following fertilization, including the calcium wave that initiates the cortical reaction:

Development Step 2: Cleavage and Blastula Stage

After fertilization successfully activates the egg, the egg begins a series of rapid cell divisions called cleavage, illustrated below. Normal cell division occurs every 18-24 hours, but cleavage cell divisions can occur as frequently as every 10 minutes. During cleavage, the cells divide without an increase in size; that is, one large single-celled zygote divides into multiple smaller cells called blastomeres. After the cleavage has produced over 100 blastomeres, the embryo is called a blastula. The blastula is usually a spherical layer of blastomeres that are considered to be the first embryonic tissue, the blastoderm. The blastoderm surrounds a fluid-filled or yolk-filled cavity, called the blastocoel (coelum = body cavity). The blastocoel is absolutely essential for the next step of development, gastrulation, which we will discuss in the next class session.

(a) During cleavage, the zygote rapidly divides into multiple cells without increasing in size. (b) The cells rearrange themselves to form a hollow ball with a fluid-filled or yolk-filled cavity called the blastula. Image credit: OpenStax Biology a: modification of work by Gray’s Anatomy; b: modification of work by Pearson Scott Foresman, donated to the Wikimedia Foundation)

 

The very early stages of mammalian development are extremely similar to other animals. Later stages of cleavage are a little different though, resulting in a structure called the blastocyst, which has an inner cell mass and an outer cell layer called the trophoblast. The inner cell mass will go on to form the embryo, and the trophoblast will contribute to the embryonic portion of placenta and nourish the embryo.

Pre-embryonic cleavages make use of the abundant cytoplasm of the fertilized egg as the cells rapidly divide without changing the total volume. The rearrangement of the cells in the mammalian blastula to two layers, the inner cell mass and the trophoblast, results in the formation of the blastocyst. Image credit: OpenStax Anatomy & Physiology.

You can visit the Virtual Human Embryo project at the Endowment for Human Development site to step through an interactive that shows the stages of embryo development, including micrographs and rotating 3-D images.

Intrinsic vs extrinsic factors in cell specialization

The information below was adapted from Khan Academy “Introduction to development.” All Khan Academy content is available for free at www.khanacademy.org

We’ve discussed 2 of the 4 general steps in early animal development. Before we move on to step number 3 (gastrulation), let’s take a moment to discuss how cells “know” what they’re supposed to do in an embryo, whether it’s to divide, to migrate to a new location, or to begin specializing into a specific cell type.

Intrinsic (lineage) information is inherited from the mother cell, via cell division. For instance, a cell might inherit molecules that “tell” it that it belongs to the neural, or nerve cell-producing, lineage of the body.

Intrinsic information is fate-specifying information found within a cell. Image credit: Khan Academy https://www.khanacademy.org/science/biology/ap-biology/developmental-biology/development-and-differentiation/a/introduction-to-development

Extrinsic (positional) information is received from the cell’s surroundings. For instance, a cell might get chemical signals from a neighbor, instructing it to become a particular kind of photoreceptor (light-detecting neuron).

Extrinsic information is fate-specifying information received from outside of the cell. Image credit: Khan Academy https://www.khanacademy.org/science/biology/ap-biology/developmental-biology/development-and-differentiation/a/introduction-to-development

 

Determining the body axes: cytoplasmic determinants and induction

One of the fundamental principles of animal development in all animals (except sponges) is the establishment of the body axes: animal bodies have lateral-medial (left-right), dorsal-ventral (back-belly), and anterior-posterior (head-feet) axes, illustrated below.

Animals have three body axes: anterior-posterior, dorsal-ventral, and left-right. Image credit: Khan Academy https://www.khanacademy.org/science/biology/ap-biology/developmental-biology/development-and-differentiation/a/introduction-to-development

How are these axes established from a ball of apparently identical cells (the blastula)? The process is different among different lineages of animals, which body axes being heavily influenced by cytoplasmic determinants in protostomes (most invertebrates), by yolk polarity in vertebrates with large amounts of asymmetrically-distributed yolk (many fish, amphibians, reptiles, and birds), and by induction (cell-cell communication) in many mammals:

  • Cytoplasmic determinants are mRNAs or proteins found in the egg prior to fertilization (they come from mom’s genome, not the embryo). They are asymmetrically distributed, so that after the first cleavage division, the two different resulting cells have different intrinsic information, which will then lead to different cell fates. One of the best understood cytoplasmic determinants is a factor called bicoid, which is present in a concentration gradient across the unfertilized eggs of Drosophila (fruit flies). The region of the egg with the highest concentration of bicoid becomes the anterior (head) portion of the embryo, while the region with the lowest bicoid concentration becomes the posterior (tail) region of the embryo. This future cell identity of anterior vs posterior is set after the very first cleavage division, where one cell gets nearly all the bicoid and the other cell has almost none. Cytoplasmic determinants are a key feature of protostome development and some deuterostomes, but they are not present in mammalian embryos.

    The distribution of bicoid, placed in the egg by the mother’s genome, determines the anterior and posterior axis of Drosophila embryos. Adapted from CC SA 1.0, https://commons.wikimedia.org/w/index.php?curid=499601

  • Yolk polarity is a phenomenon that occurs in the eggs of animals which have large amounts of yolk in their eggs, such as amphibians, reptiles, birds, and many fish. Because the egg contains so much yolk, and the yolk is literally heavier than the rest of the egg, it sinks to the bottom of the egg due to gravity. The yolk is therefore asymmetrically distributed in the egg, and the region of the egg with very little yolk will go on to become the anterior (head) structures of the embryo, while the region of the egg containing the mass of yolk will go on to become the posterior (tail) structures of the embryo.

    The cells arising from the animal pole of amphibian eggs will go on to become the anterior (head) region of the embryo, while the cells arising from the vegetal pole will go on to become the posterior (tail) region of the embryo. Cells neare the top Image credit: Adapted from Catcasillas21 – Own work, CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=9576351

  • Induction, or cell-cell communication, is one of the five essential developmental processes, and occurs during development of all animal (and plant) species. Induction is a prime example of extrinsic information that leads to different cell fates among initially identical cells. Because mammalian embryos have no cytoplasmic determinants and have very small amounts of evenly-distributed yolk, induction is the primary process responsible for establishing the body axes in mammalian embryos.

The process of induction is important throughout development, and we will revisit it in the next class session on steps 3 and 4 of early animal development.